Beta-2-Microglobulin antibody (Biotin)

• Catalog Number: orb344771
• Category: Antibodies
• Description: Beta-2-Microglobulin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P61769
• NCBI: NP_004039.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta-2-Microglobulin Antibody biotin C
• Note: For research use only
• Application notes: Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Beta 2 Microglobulin Biotin Conjugated. Antigen: Beta-2-Microglobulin. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta-2-Microglobulin Biotin 10 µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.