Beta-2-Microglobulin antibody (Biotin)

• Catalog Number: orb344770
• Category: Antibodies
• Description: Beta-2-Microglobulin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P61769
• NCBI: NP_004039.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta-2-Microglobulin Antibody biotin C
• Note: For research use only
• Application notes: Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Beta 2 Microglobulin Biotin Conjugated. Antigen: Beta-2-Microglobulin. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta-2-Microglobulin Biotin 10 µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.