Beta-2-Microglobulin antibody (Peroxidase)

• Catalog Number: orb344744
• Category: Antibodies
• Description: Beta-2-Microglobulin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, IF: 1:200, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.
• Conjugation: HRP
• UniProt ID: P61769
• NCBI: NP_004039.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Beta-2-Microglobulin Antibody Peroxida
• Note: For research use only
• Application notes: Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable for IF and IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.

Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.

Western Blot of Peroxidase conjugated Rabbit Anti-Beta-2-Microglobulin primary antibody. Lane 1: Beta-2-Microglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 12 kDa, 12 kDa for Beta-2-Microglobulin. Other band(s): None.