Beta-2-Microglobulin antibody (Peroxidase)

• Catalog Number: orb344743
• Category: Antibodies
• Description: Beta-2-Microglobulin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, IF: 1:200, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.
• Conjugation: HRP
• UniProt ID: P61769
• NCBI: NP_004039.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Beta-2-Microglobulin Antibody Peroxida
• Note: For research use only
• Application notes: Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable for IF and IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.

Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.

Western Blot of Peroxidase conjugated Rabbit Anti-Beta-2-Microglobulin primary antibody. Lane 1: Beta-2-Microglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 12 kDa, 12 kDa for Beta-2-Microglobulin. Other band(s): None.