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Beclin 1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb2563484

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb2563484
CategoryAntibodies
DescriptionBeclin 1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant human Beclin 1 protein (141-450/450aa)
Dilution rangeIHC-P=1:50-200, WB=1:100-1000, IF=1:50-200, IHC-F=1:50-200
Form/AppearanceLiquid
ConjugationUnconjugated
MW50 kDa
TargetBECN1
UniProt IDQ14457
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesBeclin-1; ATG6; beclin1; VPS30; Beclin 1 like prot
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NoteFor research use only
Expiration Date12 months from date of receipt.
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Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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25 ug total protein per lane of various lysates probed with Beclin 1 monoclonal antibody, unconjugated (orb2563484) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Human Kdiney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Beclin 1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.