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Catalog Number | orb2563484 |
---|---|
Category | Antibodies |
Description | Beclin 1 Recombinant Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant human Beclin 1 protein (141-450/450aa) |
Dilution range | IHC-P=1:50-200, WB=1:100-1000, IF=1:50-200, IHC-F=1:50-200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 50 kDa |
Target | BECN1 |
UniProt ID | Q14457 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like prot Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
20 ng rhBeclin 1-His protein per lane probed with Beclin 1 polyclonal antibody respectively, unconjugated (orb2563484) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates probed with Beclin 1 monoclonal antibody, unconjugated (orb2563484) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Kdiney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.