Beclin 1 Antibody (monoclonal, 2D12A3)

• Catalog Number: orb1145791
• Category: Antibodies
• Description: Beclin 1 Antibody (monoclonal, 2D12A3)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2D12A3
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 52-60 kDa
• UniProt ID: Q14457
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of human breast cancer tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of human colonic adenoma tissue.

IF analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in an immunocytochemical section of HepG2 cells.

Western blot analysis of Beclin 1 using anti-Beclin 1 antibody.

Flow Cytometry analysis of PC-3 cells using anti-Beclin 1 antibody(Blue line).Isotype control antibody(Green line) was mouse IgG.Unlabelled sample(Red line) was also used as a control.