BCL2L11 Antibody (Center)

• Catalog Number: orb1928438
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22105
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:25, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 22171 Da
• Target: This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human BCL2L11.
• UniProt ID: O43521
• NCBI: NP_001191035.1, NP_001191038.1, NP_001191037.1, NP_619528.1, NP_619529.1, NP_619527.1, NP_619531.1, NP_001191036.1, NP_001191039.1, NP_001191041.1, NP_006529.1, NP_619530.1, NP_001191040.1, NP_61953, NP_001191042.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BCL2L11 Antibody (Center) in K562, HL-60 cell line lysates (35 ug/lane). BCL2L11 (arrow) was detected using the purified Pab.

Flow cytometric analysis of WiDr cells using BCL2L11 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL2L11 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with BCL2L11 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.