BCL2L11 Antibody (Center)

• Catalog Number: orb1788197
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 134-160 aa
• Dilution range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 22171 Da
• Target: This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human BCL2L11.
• UniProt ID: O43521
• NCBI: NP_001191035.1, NP_001191038.1, NP_001191037.1, NP_619528.1, NP_619529.1, NP_619527.1, NP_619531.1, NP_001191036.1, NP_001191039.1, NP_001191041.1, NP_006529.1, NP_619530.1, NP_001191040.1, NP_61953, NP_001191042.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BCL2L11; BIM; Bcl-2-like protein 11; Bcl2-interact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of WiDr cells using BCL2L11 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL2L11 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from K562, A431 cell line (from left to right), using BCL2L11 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with BCL2L11 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.