BCL2L1 Antibody

• Catalog Number: orb1252823
• Category: Antibodies
• Description: BCL2L1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BX006
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG2a
• Immunogen: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. This antibody reacts with both BclXS and BclXL.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BCL2L1
• UniProt ID: Q07817
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Bcl-xL Antibody: BCLX, BCL2L, BCLXL, BCLXS, Bcl-X,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. This antibody reacts with both BclXS and BclXL.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of Hodgkin's lymphoma stained with Bcl-X antibody (BX006). Note cytoplasmic and membrane staining.

Western blot testing of human samples.

SDS-PAGE Analysis of Purified, BSA-Free Bcl-X Antibody (clone BX006). Confirmation of Integrity and Purity of the Antibody.