BCL2 Antibody

• Catalog Number: orb1239256
• Category: Tools
• Description: BCL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: Anti-Bcl-2 antibody (orb1239256) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2μg/mL; IHC-P: 2 μg/mL; IF: 10 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples ; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26kDObserved: 26 kD
• Target: BCL2
• UniProt ID: P10415
• NCBI: AAH27258
• Storage: Bcl-2antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulato
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Bcl-2 orb1239256, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: Human Daudi cells, Lane B: Mouse A-20 cells.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Bcl-2 orb1239256, (2 µg/mL), Bcl-2 orb1239055, (2 µg/mL), beta-actin (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Bcl-2 orb1239256, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (orb1239256) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Bcl-2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with orb1239256 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody (orb1239256) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Bcl-2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with orb1239256 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).