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Catalog Number | orb606958 |
---|---|
Category | Antibodies |
Description | Bcl10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of Bcl10 was shown to induce NFkB activation in a NIK-dependent pathway. This antibody labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BL10/2988R |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A portion of amino acids 122-168 from the human protein was used as the immunogen for this recombinant Bcl10 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Bcl10 antibody is available for research use only. |
Entrez | 8915 |
Storage | Store the recombinant Bcl10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Bcl10 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent staining of PFA-fixed human K562 cells with recombinant Bcl10 antibody (clone BL10/2988R, green) and phalloidin (red).
IHC staining of FFPE human tonsil with recombinant Bcl10 antibody (clone BL10/2988R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry staining of PFA-fixed human K562 cells with recombinant Bcl10 antibody; Red=isotype control, Blue= recombinant Bcl10 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant Bcl10 antibody (clone BL10/2988R) as confirmation of integrity and purity.
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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