Bcl-2 Antibody

• Catalog Number: orb750150
• Category: Antibodies
• Description: Bcl2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ARBC2-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Bcl2 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Bcl2 antibody is available for research use only.
• UniProt ID: P10415
• Storage: Store the Bcl2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Bcl2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human melanoma with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

IHC testing of FFPE human tonsil with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

Western blot testing of human MCF7 cell lysate with Bcl2 antibody (clone ARBC2-1). Routinely observed at 18-22 kDa.