BCKDHA, Phospho (S292) Antibody

• Catalog Number: orb1522551
• Category: Antibodies
• Description: BCKDHA, Phospho (S292) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 292
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BCKDHA, Phospho (S292)
• UniProt ID: P12694
• NCBI: NP_000700.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Alternative names: BCKDH E1-alpha; OVD1A; MSUD1; MSU; branched chain
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human pBCKDHA (S292) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells treated with phosphatase (+) or mock treated (-). Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-BCKDHA antibody.

Detection of human pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.