Bax Mouse Polyclonal Antibody

• Catalog Number: orb10172
• Category: Antibodies
• Description: Bax Mouse Polyclonal Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bax (84-175/192aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BAX
• UniProt ID: Q07812
• RRID: AB_10748744
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: apoptosis regulator BAX; Apoptosis regulator BAX c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Recombinant human Bax Protein (full length) Overexpression E.coli Lysate at 2 ug Primary: Anti-Bax (orb10172) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 73 kD.

Sample: Placenta (Mouse) Lysates, 30 ug, Primary: Anti-Bax (orb10172) at 1:300, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 21 kD, Observed band size: 23 kD.

Tissue/cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (orb10172) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Bax Polyclonal Antibody, Unconjugated (orb10172) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (orb10172) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.