Bax Antibody

• Catalog Number: orb749298
• Category: Antibodies
• Description: This antibody recognizes a protein of 21kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2D2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Bax antibody is available for research use only.
• Entrez: 581
• Storage: Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE Hodgkin's lymphoma stained with Bax antibody (clone 2D2).

Immunofluorescent staining of fixed human Jurkat cells with Bax antibody (clone 2D2, green) and NucSpot nuclear stain (red).

Flow cytometry testing of fixed human Jurkat cells with Bax antibody (clone 2D2); Gold=isotype control, Blue=Bax antibody.

SDS-PAGE analysis of purified, BSA-free Bax antibody (clone 2D2) as confirmation of integrity and purity.