Bax Antibody (BH3 Domain Specific)

• Catalog Number: orb31066
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BAX
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0952
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:2000, IHC-P: 1:50-100, FACS: 1:10-50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 21184
• Target: BAX
• UniProt ID: Q07812
• NCBI: NP_004315.1, NP_620119.2, NP_620118.1, NP_620116.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: anti-Apoptosis regulator BAX antibody, anti- BAX a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: THP-1 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: L929 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: Raji whole cell lysate. Lane 2: SH-SH5Y whole cell lysate. Lane 3: HT-1080 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: L929 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: L929 whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Bax Antibody (BH3) at 1:2000 dilution + mouse spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.