Basic Cytokeratin Antibody

• Catalog Number: orb606563
• Category: Antibodies
• Description: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRTH/2147R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for the recombinant Basic Cytokeratin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2 ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Basic Cytokeratin antibody is available for research use only.
• UniProt ID: Q01546
• Storage: Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with recombinant Basic Cytokeratin antibody (green, clone KRT18/834) and Reddot nuclear stain (red).

IHC testing of FFPE human prostate cancer with recombinant Basic Cytokeratin antibody (clone KRTH/2147R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Basic Cytokeratin antibody (clone KRTH/2147R) as confirmation of integrity and purity.