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Catalog Number | orb1825733 |
---|---|
Category | Antibodies |
Description | This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa. mAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rKRTH/8883 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Recombinant full-length human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This recombinant Basic Cytokeratin antibody is available for research use only. |
UniProt ID | Q01546 |
Storage | Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human skin tissue with recombinant Basic Cytokeratin antibody (clone rKRTH/8883). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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