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Catalog Number | orb1256198 |
---|---|
Category | Antibodies |
Description | BAG2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 24kDa |
Target | BAG2 |
UniProt ID | O95816 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | BAG2, RP3-496N17.2, BAG-2, KIAA0576, MGC149462, dJ Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using BAG2 antibody (orb1256198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human kidney using BAG2 antibody (orb1256198) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BAG2 antibody (orb1256198) at dilution of 1:100. Blue: DAPI for nuclear staining.
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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