B7-H3 Detection Set (Risk Free)

• Catalog Number: orb1227437
• Category: Assays and Kits
• Description: B7-H3 Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: B7-H3 antibodies were raised against the extracellular domain of human B7-H3
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with orb1240155, orb1239243, orb1239244, orb1239247, and orb1239231 antibodies at decreasing concentrations.

Western blot analysis of B7-H3 in HEK293 cells using orb1240155, orb1239243, and orb1239244 antibodies at (A) 0.25 mg/ml, (B) 0.5 mg/ml, and (C) 1 μg/ml.

Immunocytochemistry of B7-H3 in HEK293 cells using (A) orb1240155, (B) orb1239243, (C) orb1239244, (D) orb1239247, (E) orb1239231, and (F) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of B7-H3 in HEK293 cells using (A) orb1240155, (B) orb1239243, (C) orb1239244, (D) orb1239247, (E) orb1239231, and (F) control mouse IgG antibody at 5 μg/ml.

Immunofluorescence of B7-H3 in human colon carcinoma tissue using (A) orb1239243, (B) orb1239244, (C) orb1239247, (D) orb1239231, and (E) control mouse IgG antibody at 10 μg/ml.

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using (A) orb1240155, (B) orb1239243, (C) orb1239244, (D) orb1239247, (E) orb1239231, and (F) control mouse IgG antibody at 2 μg/ml.

Flow cytometry analysis of B7-H3 in HEK293 cells using (A) orb1240155, (B) orb1239243, (C) orb1239244, (D) orb1239247, and (E) orb1239231 at 1 μg/ml. Blue: isotype control on HEK293 cells. Yellow: B7-H3 mAb on HEK293 cells.