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Catalog Number | orb1231268 |
---|---|
Category | Proteins |
Description | B7-H2 / ICOSLG Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Dilution range | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 53.1 kDa |
Target | ICOSLG |
UniProt ID | O75144 |
Protein Sequence | Asp 19 - Ser 258 |
Source | HEK293 cells |
NCBI | NP_056074.1 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | ICOSLG,B7-H2,B7H2,B7RP-1,B7RP1,CD275,GL50,ICOS-L,I Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human B7-H2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ICOS (C136S, C137S), His Tag at 5 ug/ml (100 uL/well) can bind Human B7-H2, Fc Tag with a linear range of 0.3-5 ng/mL.
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
52.7 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
28.2 kDa | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
53.0 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
28.5 kDa | |
HEK293 cells |
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