B7-1 / CD80 Recombinant Protein

• Catalog Number: orb1231165
• Category: Proteins
• Description: B7-1 / CD80 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: Mouse IgG Fc Tag
• Dilution range: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SDS-PAGE.
• MW: 50.9 kDa
• Target: CD80
• UniProt ID: P33681
• Protein Sequence: Val 35 - Asn 242
• Source: HEK293 cells
• NCBI: 005182.1
• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH 7.5
• Alternative names: CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
• Note: For research use only
• Application notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human CTLA-4, His Tag at 1 ug/ml (100 uL/well) can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL.

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/ml (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/ml.