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Catalog Number | orb1231165 |
---|---|
Category | Proteins |
Description | B7-1 / CD80 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Mouse IgG Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 50.9 kDa |
Target | CD80 |
UniProt ID | P33681 |
Protein Sequence | Val 35 - Asn 242 |
Source | HEK293 cells |
NCBI | 005182.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 Read more... |
Note | For research use only |
Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CTLA-4, His Tag at 1 ug/ml (100 uL/well) can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL.
Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/ml (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/ml.