B23 (phospho-Thr234) antibody

• Catalog Number: orb769287
• Category: Antibodies
• Description: Rabbit polyclonal antibody to B23 (phospho-Thr234)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr234. AA range:201-250
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 38
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NPM1 antibody, anti-NPM antibody, anti-Nucleo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NPM (Phospho-Thr234) Antibody.

Western blot analysis of lysates from HeLa cells treated with nocodazole 1ug/ml 18h, using NPM (Phospho-Thr234) Antibody. The lane on the right is blocked with the phospho peptide.