Azide-PEG3-FLAG

• Catalog Number: orb532286
• Category: Tools
• Description: Azide-PEG 3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne-or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be de-tected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solu-bility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 µM). This concentrations may serve as a starting point for individual assay set-up.
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1485.49 g/mol; Detected MW: 1484.57 g/mol
• Formula: C₅₉H₈₈N₁₆O₂₇S
• Solubility (25°C): water, PBS (up to 30 mM tested)
• Storage: store at -20 °C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.