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Catalog Number | orb1734977 |
---|---|
Category | Tools |
Description | Azide-PEG 3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne-or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be de-tected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solu-bility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 µM). This concentrations may serve as a starting point for individual assay set-up. |
Form/Appearance | solid; Color: white to off-white |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 1485.49 g/mol; Detected MW: 1484.57 g/mol |
Formula | C59H88N16O27S |
Solubility (25°C) | water, PBS (up to 30 mM tested) |
Storage | store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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