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Catalog Number | orb1262869 |
---|---|
Category | Antibodies |
Description | AURKA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This AIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-120 amino acids from the N-terminal region of human AIK. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 46 kDa |
Target | AURKA |
UniProt ID | O14965 |
NCBI | O14965 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Aurora kinase A, Aurora 2, Aurora/IPL1-related kin Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of AIK using rabbit polyclonal hAIK-H105 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AIK antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
ELISA, FC, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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