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Catalog Number | orb576251 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Atp6v0a1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Bovine, Equine, Porcine, Rabbit, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen is a synthetic peptide corresponding to a region of Mouse |
Concentration | 0.5 mg/ml |
Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Conjugation | Unconjugated |
MW | 96 kDa |
Target | Atp6v0a1 |
UniProt ID | A2A5A0 |
Protein Sequence | Synthetic peptide located within the following region: TLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDAEEPTEDEVFDFGDTMVH |
NCBI | NP_058616 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Alternative names | V, Vp, Atp, Vpp1, Vpp-1, ATP6a1, Atp6n1, Atp6n1a, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/ml of the antibody was used in this experiment.
Application: Western blotting, Species+tissue/cell type: HeLa cells, How many ug'sof tissue/cell lysate run on the gel: 1. 10 ug untransfected HeLa lysate, 2. 10 ug mATP6V0A2 (Partial) transfected HeLa lysate, 3. 10 ug mATP6V0A2-FLAG transfected HeLa lysate, 4. 10 ug mATP6V0A1-FLAG transfected HeLa lysate, Primary antibody dilution: 1:300, Secondary antibody: Anti-rabbit-HRP, Secondary antibody dilution: 1:1000.
Host: Rabbit, Target Name: Atp6v0a1, Sample Tissue: Human DLD1 Whole Cell, Antibody Dilution: 1 ug/ml.
Host: Rabbit, Target Name: Atp6v0a1, Sample Tissue: Mouse Mouse Skeletal Muscle, Antibody Dilution: 1 ug/ml.
Sample Type: A. untransfected HeLa cells, B. mATP6V0A1 transfected HeLa cells, Primary Antibody Dilution: 1:250, Secondary Antibody: Anti-rabbit AlexaFluor 488, Secondary Antibody Dilution: 1:1000, Color/Signal Descriptions: Atp6v0a1: Green DAPI:Blue, Gene Name: Atp6v0a1.
WB Suggested Anti-Atp6v0a1 Antibody Titration: 0.2-1 ug/ml, Positive Control: Mouse Uterus.
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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