ATP6AP2 Antibody

• Catalog Number: orb1247030
• Category: Antibodies
• Description: ATP6AP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SIIYRMTNQKIRMD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1). An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6(1):9-19, PMID: 27956412.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ATP6AP2
• UniProt ID: O75787
• NCBI: NP_005756.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosom
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1). An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6(1):9-19, PMID: 27956412.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247030 (2 ug/ml) of Mouse Kidney lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.

orb1247030 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).