ATP6 Antibody

• Catalog Number: orb1255955
• Category: Antibodies
• Description: ATP6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of mouse ATP6 (NP_904333.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 20kDa
• Target: ATP6
• UniProt ID: P00846
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ATP6, MT-ATP6, ATPase6, MTATP6
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ATP6 antibody (orb1255955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunofluorescence analysis of U2OS cells using ATP6 antibody (orb1255955) at dilution of 1:100. Blue: DAPI for nuclear staining.