ATP1B2 antibody

• Catalog Number: orb374297
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP1B2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-290 of human ATP1B2 (NP_001669.3).
• Dilution range: WB: 1:200-500
• Conjugation: Unconjugated
• MW: 42kDa
• UniProt ID: P14415
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: ATP1B2, AMOG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of C6 cells using ATP1B2 Rabbit pAb (orb374297) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ATP1B2 antibody (orb374297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.