ATM (phospho-S1981) antibody

• Catalog Number: orb345897
• Category: Antibodies
• Description: ATM (phospho-S1981) antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This antibody was affinity purified from whole sheep serum prepared by repeated immunizations with a synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays. This polyclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human ATM. Cross reactivity with ATM from other mammalian sources has not been tested.
• Conjugation: Unconjugated
• UniProt ID: Q13315
• NCBI: NP_000042.3
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-ATM pS1981 Antibody, AT mutated antibod
• Note: For research use only
• Application notes: Affinity purified sheep anti-ATM has been tested by ELISA and western blotting against native form phosphorylated ATM PK.
• Expiration Date: 12 months from date of receipt.

Schematic of ATM induction by DNA damage.

Western Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate (p/n orb348664). Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate (p/n orb348670). Load: 35 µg per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye™800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5000 for 1 h at room temperature. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms.