ATG5 antibody

• Catalog Number: orb33366
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB7466
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Porcine, Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IF/ICC: 1:200, IHC-P: 1:50-100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 32447
• Target: ATG5
• UniProt ID: Q9H1Y0
• NCBI: NP_004840.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-APG5 antibody, anti-APG5-LIKE antibody, anti-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Kessel, David et al. Effects of Combined Lysosomal and Mitochondrial Photodamage in a Non-small-Cell Lung Cancer Cell Line: The Role of Paraptosis Photochem Photobiol, 93, 1502-1508 (2017)

PubMed: 28696570

Ozturk, Melek et al. The Detection Techniques for Autophagy-Associated Cell Death-Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry Methods Mol Biol, 1854, 119-130 (2019)

PubMed: 28889353

Western blot analysis of 293 cell lysateusing ATG5 antibody (primary antibody at 1:1000)

Western blot analysis of 293 cell lysateusing ATG5 antibody (primary antibody dilution at: 1:1000)

Immunofluorescense analysis of U251 cells using ATG5 antibody (primary antibody dilution at: 1:200)

Immunohistochemical staining of paraffin embedded human cancer tissue using ATG5 antibody (primary antibody dilution at: 1:50-100)

Western blot analysis of Hela cell line lysates (35ug/lane)using ATG5 antibody (dilution of primary antibody at 1:1000)

Western blot analysis of mouse brain tissue lysatesusing ATG5 antibody (antibody dilution at 1:1000)