ATG5 Antibody

• Catalog Number: orb1239086
• Category: Antibodies
• Description: ATG5 Antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgY
• Immunogen: Anti-ATG5 antibody (orb1239086) was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; IHC-P: 2.5 μg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32kDObserved: 55kD (ATG5-ATG12 conjugation)
• Target: ATG5
• UniProt ID: A9UGY9
• NCBI: EAW48415
• Storage: ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation of ATG5 in Mouse Spleen. Loading: 15 µg of lysates per lane. Antibodies: ATG5, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution. Lane A: 1 µg/mL, Lane B: 2 µg/mL.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ATG5 orb1239086 (2 µg/mL), ATG5 orb1271000 (2 µg/mL), -actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.

Western Blot Validation of ATG5 in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ATG orb1239086 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of ATG5 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody (orb1239086) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-chicken IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.