ATG4B Antibody

• Catalog Number: orb1240182
• Category: Antibodies
• Description: ATG4B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.The immunogen is located within the last 50 amino acids of ATG4B.
• Concentration: 1 mg/mL
• Dilution range: ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 43 kDa Observed: 46 kDa
• Target: ATG4B
• UniProt ID: Q9Y4P1
• NCBI: NP_037457
• Storage: ATG4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: ATG4B antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Autophagy related 4B cysteine peptidase, APG4B, AP
• Note: For research use only
• Application notes: ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 µg/mL.

Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 µg/ml.

Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 µg/ml.