ATF6 Antibody

SKU: orb1239223

Description

ATF6 Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.The immunogen is located within amino acids 470 - 520 of ATF6.
• Target: ATF6
• Purification: ATF6 Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha

Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 µg/mL.

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 µg/mL.

Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 µg/ml.

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.

Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 µg/ml.

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.

Quick Database Links

Gene Symbol

ATF6

UniProt

P18850

RefSeq (Protein):NP_031374