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Item 1 of 4
ATF6 Antibody (Center)
Catalog Number: orb1928352
| Catalog Number | orb1928352 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB22035 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, WB: 1:16000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 74585 Da |
| Target | This ATF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids of human ATF6. |
| UniProt ID | P18850 |
| NCBI | NP_031374.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-6 al Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of ATF6 Antibody (Center) in A2058 cell line lysates (35 ug/lane). ATF6 (arrow) was detected using the purified Pab.
ATF6 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ATF6 Antibody (Center) at 1: 16000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
