Atf4 Rabbit Polyclonal Antibody

• Catalog Number: orb576181
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Atf4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Rat, Sheep
• Reactivity: Mouse
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: Atf4
• UniProt ID: Q5U4B2
• Protein Sequence: Synthetic peptide located within the following region: TRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVR
• NCBI: NP_033846
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: Atf-, C/AT, CREB, Atf-4, C/ATF, CREB2, CREB-2, TAX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Mouse tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~46 kDa. Protein is modified by phosphorylation.

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.

Sample Tissue: Mouse Mouse Spleen, Antibody Dilution: 2 ug/ml.

Mouse organ of Corti labeled with orb576181 (anti-ATF4) in green, rhodamine phalloidin in red and DAPI in blue. Antibody dilution: 1:100.

WB Suggested Anti-Atf4 Antibody Titration: 0.2-1 ug/ml, Positive Control: Mouse Brain.