ATF4 Rabbit Polyclonal Antibody

• Catalog Number: orb576180
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATF4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse ATF4
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 42kDa
• Target: ATF4
• UniProt ID: Q61328
• Protein Sequence: Synthetic peptide located within the following region: MALFTKSSSSVAVTDKDTFELSTFLESSKAPQHDRDELPEQRSVGGGLDD
• NCBI: NP_033846
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: Atf-, C/AT, CREB, Atf-4, C/ATF, CREB2, CREB-2, TAX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.

Sample Tissue: Mouse Skeletal Muscle, Antibody Dilution: 3 ug/ml.

Immunohistochemistry with Human Braun, cerebellum tissue at an antibody concentration of 5.0 ug/ml using anti-ATF4 antibody (orb576180).

Rabbit Anti-ATF4 Antibody, Catalog Number: orb576180, Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue, Observed Staining: Cytoplasm, Nucleus, Primary Antibody Concentration: 1:600, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-ATF4 Antibody Titration: 0.125 ug/ml, ELISA Titer: 1:62500, Positive Control: NIH/3T3 cell lysate.