ATF2 Antibody

• Catalog Number: orb1258251
• Category: Antibodies
• Description: ATF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF2 (NP_001871.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: ATF2
• UniProt ID: P15336
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ATF2, HB16, CREB2, TREB7, CREB-2, CRE-BP1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ATF2 antibody (orb1258251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using ATF2 antibody (orb1258251) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ATF2 antibody (orb1258251) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using ATF2 antibody (orb1258251). Blue: DAPI for nuclear staining.