ASRGL1 Antibody

• Catalog Number: orb1246753
• Category: Antibodies
• Description: ASRGL1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: EKHEKGAQKTDCQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ASRGL1
• UniProt ID: Q7L266
• NCBI: NP_079356.3, NP_001077395.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ASRGL1, ALP, asparaginase like 1, ALP1, FLJ22316,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1246753 (0.5 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.