ASRGL1 Antibody / ALP1

• Catalog Number: orb750018
• Category: Antibodies
• Description: ASRGL1 (Asparaginase-like protein 1), also known as CRASH, belongs to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CRASH/1289
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human partial recombinant protein was used as the immunogen for this ASRGL1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ASRGL1 antibody is available for research use only.
• Entrez: 80150
• Storage: Store the ASRGL1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ASRGL1 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human fallopian tube with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human uterus with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free ASRGL1 Antibody (clone CRASH/1289). Confirmation of Integrity and Purity of the Antibody.