ARSA Antibody

• Catalog Number: orb1246754
• Category: Antibodies
• Description: ARSA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-YDLSKDPGENYN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 53.6kDa according to human NP_000478.2 and 53.7kDa according to Mouse NP_033843.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ARSA
• UniProt ID: P15289
• NCBI: NP_000478.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ARSA, arylsulfatase A, MLD, cerebroside-sulfatase,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 53.6kDa according to human NP_000478.2 and 53.7kDa according to Mouse NP_033843.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246754 (0.5 ug/ml) staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246754 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246754 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.