ARMENIAN HAMSTER IgG (H&L) (GOAT) antibody (Peroxidase)

• Catalog Number: orb348064
• Category: Antibodies
• Description: ARMENIAN HAMSTER IgG (H&L) (GOAT) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Hamster
• Isotype: IgG
• Immunogen: Armenian Hamster IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Greatly diminished reactivity will occur against Golden Syrian Hamster IgG.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Armenian Hamster IgG Antibody peroxidase
• Note: For research use only
• Application notes: Anti-Hamster IgG HRP Secondary Antibody has been tested by ELISA and is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Goat anti-Armenian Hamster IgG Antibody tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n orb346446). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.