Arginase antibody (Peroxidase)

• Catalog Number: orb344867
• Category: Antibodies
• Description: Arginase antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Arginase [Bovine Liver]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver]. Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: Q2KJ64
• NCBI: NP_001039619.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Arginase Antibody Peroxidase Conjugati
• Note: For research use only
• Application notes: Anti-Arginase Peroxidase Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Arginase Peroxidase Conjugated. Antigen: Arginase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Arginase HRP 10 µg/mL for 1 hr at RT. Secondary Antibody: none.