Arginase antibody

• Catalog Number: orb344796
• Category: Antibodies
• Description: Arginase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Arginase [Bovine Liver]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: Anti-Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver]. Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: Q2KJ64
• NCBI: NP_001039619.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Arginase Antibody, A I antibody, Al an
• Note: For research use only
• Application notes: Arginase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Arginase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500,000 to 1:2,000,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot results of Rabbit Anti-Arginase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: Arginase. Load: 50 ng. Primary Antibody: Rabbit Anti-Arginase at 1 µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.