Arginase 1 Antibody

• Catalog Number: orb749619
• Category: Antibodies
• Description: This protein is involved in the pathway that synthesizes L-ornithine and urea from L-arginine.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ARG1/1126
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG3, kappa
• Immunogen: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Arginase antibody is available for research use only.
• UniProt ID: P05089
• Storage: Store the Arginase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Arginase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase antibody (ARG1/1126). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT prior to testing.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Arginase antibody (clone ARG1/1126). These results demonstrate the foremost specificity of the ARG1/1126 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Arginase antibody (clone ARG1/1126) as confirmation of integrity and purity.

Western blot analysis of human liver lysate using Arginase 1 antibody (ARG1/1126). Predicted molecular weight ~35 kDa.