Arginase 1 Antibody Cocktail

• Catalog Number: orb749620
• Category: Antibodies
• Description: Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ARG1/1125 + ARG1/1126
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG3, kappa
• Immunogen: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody cocktail.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Arginase 1 antibody cocktail is available for research use only.
• UniProt ID: P05089
• Storage: Store the Arginase 1 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Arginase 1 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase 1 antibody (ARG1/1125 + ARG1/1126).