You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb750569 |
---|---|
Category | Antibodies |
Description | ARG1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine |
Isotype | Antiserum |
Immunogen | Arginase [Bovine Liver] |
Concentration | 80 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Arginase [Bovine Liver]. Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined. |
Conjugation | Unconjugated |
UniProt ID | Q2KJ64 |
NCBI | NP_001039619.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Arginase Antibody, A I antibody, Al an Read more... |
Note | For research use only |
Application notes | Anti-Arginase has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Arginase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:80,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit Anti-Arginase antibody. Lane 1: Arginase. Load: 50 ng per lane. Primary antibody: Arginase primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 35 kDa, 39 kDa for Arginase. Other band(s): Arginase splice variants and isoforms.
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine | |
Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating