ARG1 Antibody

• Catalog Number: orb1252048
• Category: Antibodies
• Description: ARG1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: T1ARG-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG3, kappa
• Immunogen: A C-terminal recombinant protein fragment from ARG1 was used as the immunogen for the Arginase 1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Arginase 1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ARG1
• UniProt ID: P05089
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Arginase-1, Liver-type arginase, Type I arginase,
• Note: For research use only
• Application notes: Immunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Arginase 1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human hepatocellular carcinoma and Arginase 1 antibody.

Western blot testing of 1) partial recombinant ARG1 protein and 2) human liver lysate using Arginase 1 antibody.