ARFGAP3 antibody

• Catalog Number: orb750534
• Category: Antibodies
• Description: ARFGAP3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
• Concentration: 70mg/mL
• Dilution range: ELISA: 1:5,000, IF: 1:100, IP: User Optimized, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-ArfGAP3 antibody was prepared from monospecific antiserum by delipidation and defibrination. Further purification was used to remove the GST tag. The antibody detects ArfGAP3 in cell lysates. A BLAST analysis was used to suggest cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross-reactivity with AfGAP3 from other sources have not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q9NP61
• NCBI: NP_001135765.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: rabbit anti-ArfGAP3 Antibody, ARFGAP1, ADP-ribosyl
• Note: For research use only
• Application notes: ArfGAP3 has been tested for use in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.

Western Blot of Rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 µg per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50 kDa.